Sodium Fluorescein / Uranine: fluorescent tracer for hydrological tracing

Sodium Fluorescein / Uranine: fluorescent tracer for hydrological tracing

Sodium fluorescein, also known as uranine, is a benchmark fluorescent tracer for visualizing natural flows, tracking water movement and carrying out hydrological studies with great precision. Visible under UV light and even at low concentration in daylight, it allows mapping of surface and underground water networks, measuring flow velocities or identifying hydraulic connections. At Fluotechnik, we offer sodium fluorescein solutions tailored to the needs of hydrogeologists, speleologists, consulting firms and environmental professionals seeking a reliable, environmentally friendly tracer.

Precise hydrological tracing

Visually track the movement of water in rivers, karsts, aquifers and underground networks.

High visibility under UV

Intense fluorescence even at very low concentration, detectable in daylight or under a UV lamp.

Environmentally friendly

Non‑toxic, biodegradable formulations with no lasting impact on ecosystems.

Versatile use

Infiltration, dispersion studies, karst tracing, permeability tests, control of natural and industrial networks.

Expert technical support

Fluotechnik advises you on preparation, dosing and the detection equipment suited to your project.

Precise hydrological tracing

Visually track the movement of water in rivers, karsts, aquifers and underground networks.

High visibility under UV

Intense fluorescence even at very low concentration, detectable in daylight or under a UV lamp.

Environmentally friendly

Non‑toxic, biodegradable formulations with no lasting impact on ecosystems.

Versatile use

Infiltration, dispersion studies, karst tracing, permeability tests, control of natural and industrial networks.

Expert technical support

Fluotechnik advises you on preparation, dosing and the detection equipment suited to your project.

Our range of products based on sodium fluorescein / uranine

To meet the various needs of hydrological tracing, Fluotechnik offers several formulations of sodium fluorescein. Depending on the intended application, you can choose between a concentrated powder to prepare to your own dosage, a ready‑to‑use liquid solution, or specific formulations that simplify field implementation. Each product is selected to ensure effective fluorescence, rapid dissolution and compatibility with the constraints of studies in natural environments.

What is sodium fluorescein / uranine and what is it used for?

Sodium fluorescein is the sodium salt of fluorescein, better known as uranine. This water-soluble dye is distinguished by its intense green fluorescence when exposed to UV light or daylight. Thanks to this property, it is widely used as a hydrological tracer to visualize and measure the path of water in natural environments (rivers, aquifers, karst systems) as well as in technical and industrial networks. Its high visibility makes it possible to detect very small amounts and to follow a flow over long distances. Used in hydrogeology, speleology, geotechnics and environmental engineering, sodium fluorescein has become an essential tool for:

  • Mapping aquifer networks and identifying connections between injection points and springs;
  • Measuring transit times or flow velocities in aquifers and streams;
  • Studying infiltrations and recharge phenomena in soils and substrates;
  • Carrying out dispersion tests to evaluate the impact of a discharge or pollution;
  • Checking the watertightness of structures such as dikes, dams, basins or irrigation networks.

By bringing together our main uranine-based solutions, this page helps you understand the differences between the available forms and select the reference best suited to your hydrological or environmental studies.

How to use sodium fluorescein / uranine for hydrological tracing?

Sodium fluorescein is used as a tracer to follow the flow of water in a natural or artificial environment. Depending on the volumes of water to be traced and the objectives of the study, preparation and method may vary. Here are the general steps to follow:

Step 1 – Prepare and dilute the fluorescein

Weigh or measure the required amount of dye based on the volume of water to be traced, the distance to be covered and the desired detection sensitivity. Dissolve the powder or concentrated solution in a quantity of clean water to obtain a homogeneous mixture. In hydrology, dosages often range from a few milligrams to several hundred grams depending on the scale of the tracing.

Step 2 – Inject the tracer and monitor observation points

Pour the solution into the watercourse, doline, fissure or chosen injection point. Note the time and hydrological conditions. Then monitor the resurgences, springs, piezometers or sampling points likely to receive the dye. Detection can be visual (UV lamp, daylight observation) or instrumental (fluorimeter, turbidity probes).

Step 3 – Interpret the results and analyze the flow

Depending on the arrival times, measured concentrations and fluorescence intensity, determine the flow velocities, transiting volumes and existing connections between the points. This analysis makes it possible to identify recharge zones, understand how an aquifer works or check the effectiveness of a hydraulic structure. Feel free to repeat the operation under different conditions to refine your conclusions.

Why choose Fluotechnik sodium fluorescein?

Choosing Fluotechnik’s sodium fluorescein solutions means benefiting from a reliable tracer and support tailored to your hydrological challenges. Our references combine performance, safety and ease of use to facilitate your investigations in the field.

Purity and controlled quality

our fluoresceins are selected and tested to guarantee optimal fluorescence and rapid dissolution.

Respect for the natural environment

all our formulations are non‑toxic, biodegradable and comply with the requirements of environmental studies.

Dosing and use advice

our team guides you on the right concentration, the right detection equipment and the procedures suited to your project.

Availability and fast delivery

we maintain regular stock and offer fast shipping so you can intervene without delay on your sites and studies.

Experience and field feedback

professionals in hydrogeology and speleology use our products daily and attest to their effectiveness.

Sodium fluorescein in video: see the tracer in real conditions

Discover through images how sodium fluorescein is used for hydrological tracing, speleology or dispersion tests in a natural environment. These videos show the implementation of the dye, its diffusion in water and the reading of results, whether with the naked eye or through fluorescence sensors. They illustrate best practices in preparation, injection and monitoring, and show how this tracer helps to better understand the functioning of aquifers and hydraulic networks.

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Need technical expertise?

Discover our Hydrology & Fluorescent Tracing catalogue: a complete selection of professional solutions dedicated to tracing water flows, studying underground networks and detecting hydraulic transfers. Fluorescent dyes, fluorimeters, detection equipment and technical accessories...

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FAQ on sodium fluorescein / uranine and its uses

Sodium fluorescein, often called uranine, is the sodium salt of fluorescein. Soluble in water, it exhibits very intense green fluorescence. This property makes it valuable for following flows or highlighting the presence of water in various environments.

In hydrology and speleology, uranine is used to trace water circulation and study the connections between different points (karst system, aquifer, resurgence). It allows transit times to be measured, flow velocities to be determined and recharge or loss zones within a catchment area to be identified.

The dosage depends on the volume of water to be traced, the distance to be covered and the detection method (visual or instrumental). Generally, a few milligrams per litre of water are used for small tests, up to several hundred grams for large river tracings. It is advisable to carry out a preliminary test or consult our recommendations to adapt the quantity to your context.

At appropriate dosages, sodium fluorescein is considered low‑toxic and biodegradable. It is used in many environmental studies without lasting impact. However, it is advisable to comply with local regulations and limit concentrations to avoid persistent coloration or visual disturbance.

Fluorescein in its acid form (fluorescein free acid) is poorly soluble in water, whereas sodium fluorescein is the salified form that dissolves easily. Uranine is therefore better suited to aquatic tracing. It should not be confused with non‑fluorescent colored tracers, such as food colorings.

In addition to sodium fluorescein, Fluotechnik already offers or will soon offer tracers based on eosin, rhodamine WT, sulforhodamine B and other specific dyes (Tinopal CBS‑X, amino‑G acid, etc.). Each tracer has its own color and characteristics, enabling multiple tests without interference or adaptation to particular conditions.

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Choosing the right sodium fluorescein for your use

Sodium fluorescein is now indispensable for studies where visualizing water flows is essential. In hydrology, geology or environmental work, it offers a simple way to observe circulations, confirm hypotheses and quantify exchanges. The choice of formulation will mainly depend on:

  • The nature of the study: karst tracing, checking the watertightness of a structure, dispersion or pollution study;
  • The volume and the environment: small volumes in the laboratory, underground networks or large rivers;
  • The detection method: visual observation under UV, samples for fluorimeter analysis or continuous monitoring with probes.

Based on these criteria, you will choose a concentrated powder to dilute, a ready‑to‑use liquid solution or a specific dye. The goal remains to guarantee sufficient fluorescence to obtain reliable data while limiting impact on the environment. By bringing together our main references on this page, Fluotechnik helps you compare the possibilities, understand the advantages of each product and select the one best suited to your hydrological project.

To go further, you can consult our other tracing substances (eosin, rhodamines, sulforhodamines) as well as our technical advice on dosing, the use of fluorimeters and designing an effective tracing plan. Our team is available to answer your questions and support you in your studies.

Discover other tracing solutions for hydrology and the natural environment

Depending on the environment being studied, the fluorescence colour required, the detection method and the type of monitoring implemented, sodium fluorescein / uranine is not always the only relevant solution. Fluotechnik helps you guide your project towards the right fluorescent tracer, the right measuring equipment or the right observation method according to your field context, your study objectives and the level of precision required.

Rhodamine WT 20%

A benchmark fluorescent tracer for certain hydrological monitoring projects, dispersion tests and flow studies.
Rhodamine WT 20%
Rhodamine WT is especially valued in tracing projects where a different fluorescent signature, good stability or compatibility with certain measurement protocols is required. Discover this solution for water circulation studies and field testing in natural environments.
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Eosin

A useful fluorescent tracer for certain hydrological studies, flow tests and observations in natural environments.
Eosin
Depending on the nature of the environment, the fluorescence colour required or the detection constraints, eosin can be an interesting alternative to sodium fluorescein. Discover this tracing substance used in various flow studies and hydrological monitoring contexts.
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Sulforhodamine B

For tracing projects requiring a different fluorescence colour or multi-tracer tests without interference.
Sulforhodamine B
When a study requires distinguishing several water flows or working with multiple fluorescent substances, sulforhodamine B can be an excellent complement. Discover this tracer for comparative approaches, multi-tracer tests and certain environmental monitoring projects.
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Fluorometers

To detect, measure and precisely monitor fluorescence in the field or during the analysis phase.
Fluorometers
Sodium fluorescein reveals its full potential when combined with suitable measuring equipment. Discover our fluorometers to improve detection, quantify concentrations and strengthen the interpretation of results during your hydrological and environmental studies.
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Data loggers

To monitor field parameters, structure measurement campaigns and secure the interpretation of results.
Data loggers
In hydrological tracing projects, the quality of monitoring also depends on proper data acquisition. Discover our data loggers to complement your measuring systems, track parameter evolution and make better use of your field observations.
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All our tracers

To compare all our tracing substances according to colour, application, environment studied and detection method.
All our tracers
Not every tracing project uses the same substance. If you want to compare several solutions before choosing the one best suited to your study, discover our full tracer range for hydrology, natural environments, flow testing and environmental monitoring.
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